Suite2p GUI

Section 1: Installation And Data Access

conda create --name s2p python=3.9
conda activate s2p
pip install "suite2p[gui,io]"
suite2p

Download files from the following links and place them in separate folders

1. demoMovie.tif
2. data.tif

Section 2: Exercise 1

1. Load demoMovie.tiff
2. Run suite2p pipeline with default parameters
3. Examine the following
   1. Open both Cells and Not-Cells
   2. Flip an roi from cell to not-cell (Right click)
   3. Flip a not-cell to cell (Right click)
   4. Click on an ROI and examine the calcium traces, neuropil traces, and deconvolved traces.

Section 3: Exercise 2

1. Load demoMovie.tiff
2. Run suite2p pipeline with the following parameters
   1. tau = 0.7
   2. fs = 30
3. Examine the following
   1. Open both Cells and Not-Cells
   2. Flip an roi from cell to not-cell (Right click)
   3. Flip a not-cell to cell (Right click)
   4. Click on an ROI and examine the calcium traces, neuropil traces, and deconvolved traces.

Section 4: Exercise 3

1. Load data.tiff
2. Run suite2p pipeline with the following parameters
   1. tau = 0.7
   2. fs = 30
3. Examine the following
   1. Open both Cells and Not-Cells
   2. Flip an roi from cell to not-cell (Right click)
   3. Flip a not-cell to cell (Right click)
   4. Click on an ROI and examine the calcium traces, neuropil traces, and deconvolved traces.